All medical professionals should use a comprehensive approach for providing care of patients with head injuries. This study was designed to explore the knowledge and practice of the nurses regarding the care of head injury patients in Bangladesh. This cross-sectional study was conducted among 288 respondents at Dhaka Medical College Hospital from January 2020 to June 2020 by using systematic random sampling technique. Statistical tests, including descriptive statistics and the χ2 test, were used to analyze associations between variables. The p-value level of 0.05 or below was considered to test statistical significance. Study findings showed that most of the participants 120 (75.9%) were in between 21-30 years of age group and majority (37.54%) of the nurses have working experience of 11 to 15 years. Among all the respondents, 81.15% had excellent level of knowledge and 85.63% had excellent level of practice on care of head injury. There is a significant association of age group, level of education and year of experience with the level of knowledge and sex, level of education was statistically significant with the level of practice among the nurses regarding care of head injury patients (p-value<0.05). The study findings suggested the development of a system for periodic evaluation of nurses to identify strategies for updating their knowledge and enhancing their practice.