Salbutamol sulphate is a short-acting β2-adrenergic receptor agonist used for the relief of bronchospasm in conditions such as asthma and chronic obstructive pulmonary disease (COPD). It is widely produced and marketed drug by many pharmaceutical companies in Bangladesh. Five different brands of salbutamol sulphate syrup manufactured by Bangladeshi pharmaceutical companies were collected from different retail pharmacy of Dhaka and Noakhali, Bangladesh. The performance evaluation (appearance, organoleptic properties, density, viscosity, pH, potency and microbial count) was carried out in order to find out whether they really comply with the required standards. The results of viscosities, density, and pH of the entire sample were within the official specification. The potency of the representative samples was determined by uv-spectrophotometric method, result of all brands were 96.07 % to 104.5% which is within the official limit. This study was also conducted to determine the microbiological quality of different brands. Microbiological analysis was carried out using spread plate technique on culture media. This study gives the idea about the overall quality of salbutamol sulphate syrup, manufactured by pharmaceutical companies of Bangladesh.